Home Print this page Email this page Users Online: 3506
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Year : 2015  |  Volume : 23  |  Issue : 1  |  Page : 60-64

Prevalent resistance mechanisms in isolates from patients with burn wounds

1 Department of Microbiology, Government Medical College Hospital, Chandigarh, Punjab, India
2 Department of General Surgery, Government Medical College Hospital, Chandigarh, Punjab, India

Correspondence Address:
Dr. Manpreet Kaur
Department of Microbiology, Government Medical College Hospital, Sector 32, Chandigarh, Punjab
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0971-653X.171659

Rights and Permissions

Context: Infections are the major cause of morbidity and mortality in burn patients. The Extended Spectrum Beta Lactamase (ESBL), AmpC Beta Lactamase (AmpC), and Metallo-Beta Lactamase (MBL) are the major mediators of antimicrobial resistance in Gram-negative organisms. Methicillin-resistant Staphylococcus aureus (MRSA) are also implicated in causing serious infections in burn patients. Aim: To find the prevalence of ESBL, AmpC, and MBL mediated resistance among Gram-negative organisms and MRSA in Staphylococcus aureus isolates in pus samples obtained from the burn unit. Materials and Methods: ESBL, Amp C, and MBL production was detected using ceftazidime and ceftazidime-clavulanic acid combination disc test, cefoxitin and cefoxitin/boronic acid disk test, and Imipenem-EDTA disk test, respectively. MRSA were screened using oxacillin disc by disc-diffusion technique. Results: High ESBL rate (37%) was seen among Escherichia coli isolates, whereas Acinetobacter cbc exhibited maximum (25%) MBL activity. Among Klebsiella pneumoniae isolates, 20% isolates were ESBL and AmpC producers, whereas no AmpC expression and no co-existence of these enzymes were seen in Escherichia coli. Co-existence of AmpC with MBLs was seen in 9.6% of Pseudomonas aeruginosa isolates while ESBL expression alone was seen in 16.1% of isolates. Conclusions: Drug resistance to antimicrobial agents is a serious threat in burn infection. Early detection of these β-lactamase producing isolates in a diagnostic laboratory could help to avoid treatment failure, as often the isolates producing this enzyme show a susceptible phenotype in routine susceptibility testing.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded172    
    Comments [Add]    
    Cited by others 2    

Recommend this journal